Produção de biomassa e estrutura do pasto de capim-andropogon em sistema silvipastoril e monocultura / Biomass production and pasture structure of Andropogon grass in a silvopastoral system and in monoculture

A produção de biomassa e as características estruturais do capim-andropogon foram avaliadas nas idades de rebrota de 35, 49 e 63 dias, em três ambientes de um sistema silvipastoril: sob a copa de pau-d'arco, sob a copa de jatobá, em área aberta; local fora da copa de qualquer árvore e em sistema de monocultura. O delineamento experimental foi o de parcelas subdivididas com cinco repetições. As parcelas foram representadas pelos ambientes e as subparcelas, pelas idades de rebrota. A luminosidade sob pau-d'arco, sob jatobá e em área aberta foi de 74, 62 por cento e 82 por cento, respectivamente, das áreas a pleno sol. Houve interação ambiente versus idade de rebrota. O sombreamento sob a copa de pau-d'arco e de jatobá não afetou a produção de matéria seca do capim-andropogon, contudo reduziu a altura do pasto aos 63 dias de rebrota. O percentual de folhas da forragem aos 35 dias de rebrota foi mais elevado na monocultura. Nas outras idades de rebrota, os quatro ambientes não diferiram entre si.

The biomass production and pasture structure of Andropogon grass were evaluated at 35, 49, and 63 days of regrowth, in three silvopastoral environments: under Pau-d'Arco and Jatobá trees, in open field and in monoculture system. The experiment was designed in split plot with five replications, the plots were the environments and the split plots were the regrowth ages. The luminosities under Pau-d'Arco, Jatobá, and open field were: 74, 62, and 82 percent, respectively, relative to the areas under full sun. There was interaction among environments and ages of regrowth. The shading under the canopy of Pau-d'Arco and Jatobá did not affect the production of dry matter of Andropogon grass; however, reduced the height of the grass at 63 days of regrowth. The percentage of leaves of forage at 35 days of regrowth was higher in monoculture. At other ages of regrowth, the four environments did not differ among themselves.

L1 sequence of a new human papillomavirus type-58 variant associated with cervical intraepithelial neoplasia.

The present study on molecular characterization of a human papillomavirus (HPV) isolated in Central Brazil describes the L1 gene sequence from a new variant of HPV-58, the isolate Bsb-02. The sample was from a smear obtained from a woman with cervical intraepithelial neoplasia grade II. The whole L1 gene from isolate Bsb-02 was sequenced automatically, showing 99.1% nucleotide identity with the gene from the HPV-58 reference. The clustering between Bsb-02 and HPV-58 reference sequence was also supported by phylogenetic analysis. Fourteen nucleotide substitutions were observed: eight were synonymous and six were associated with amino acid substitutions. A10V and V144I have not been previously described. At GenBank, the only complete L1 sequence from HPV-58 in addition to the HPV-58 reference one is that of Bsb-02. These data provide information that may be relevant to HPV diagnosis and to rational vaccine strategies. HPV variants may also be associated with host immune responses and with the risk of cervical neoplasia.

L1 sequence of a new human papillomavirus type-58 variant associated with cervical intraepithelial neoplasia

The present study on molecular characterization of a human papillomavirus (HPV) isolated in Central Brazil describes the L1 gene sequence from a new variant of HPV-58, the isolate Bsb-02. The sample was from a smear obtained from a woman with cervical intraepithelial neoplasia grade II. The whole L1 gene from isolate Bsb-02 was sequenced automatically, showing 99.1 percent nucleotide identity with the gene from the HPV-58 reference. The clustering between Bsb-02 and HPV-58 reference sequence was also supported by phylogenetic analysis. Fourteen nucleotide substitutions were observed: eight were synonymous and six were associated with amino acid substitutions. A10V and V144I have not been previously described. At GenBank, the only complete L1 sequence from HPV-58 in addition to the HPV-58 reference one is that of Bsb-02. These data provide information that may be relevant to HPV diagnosis and to rational vaccine strategies. HPV variants may also be associated with host immune responses and with the risk of cervical neoplasia.